This is the on-line help file for Clustal X (version 1.8), using the NCBI Vibrant Toolkit. For full details of usage and algorithms, please read the ClustalW.DOC and ClustalV.DOC files. General help for CLUSTAL X (1.8) Input / Output Files Editing Alignments Multiple Alignments Profile and Structure Alignments Secondary Structure / Gap Penalty Masks Phylogenetic Trees Colors Alignment Quality Analysis Command Line Parameters References Toby Gibson EMBL, Heidelberg, Germany. Des Higgins UCC, Cork, Ireland. Julie Thompson/Francois Jeanmougin IGBMC, Strasbourg, France.